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最新消息活動Proteinase K Recombinant Protein 特惠促銷中

Proteinase K Recombinant Protein 特惠促銷中

Proteinase K Recombinant Protein
Code CSB-AP361972TIQ
Size                        1kg        1g        100mg        20mg                      
Image
  • SDS-PAGE

    (Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
    According to the left SDS-PAGE image, the purity of Proteinase K is 95%+ and the molecular weight is 28.9 kDa.


Product Details

Description
Proteinase K, originally isolated from the mold Tritirachium album, is a serine protease with broad substrate specificity and relatively high proteolytic activity. It preferentially cleaves ester and peptide bonds adjacent to the C-termini of hydrophobic, aliphatic, or aromatic amino acids. CUSABIO's recombinant proteinase K is highly pure determined by SDS-PAGE and reached up to 95%. It is characterized by high purity, sterility, no bio-burden, and no presence of DNAse, RNAse, DNA, and RNA contaminants. Its enzyme activity was calculated >30U/mg using the absorbance A275 as the vertical axis and different concentrations of a tyrosine as the horizontal axis. The working concentration of proteinase K, usually determined by the temperature, PH, and whether the buffer used contains SDS and urea, is 20mg/mL. This recombinant proteinase K protein is in-stock now. Proteinase K has a wide range of applications. In nucleic acid extraction and isolation, proteinase K can be used to degrade D...Read more
Purity Greater than 95% as determined by SDS-PAGE.
Activity Using the absorbance A275 as the vertical axis and different concentrations of tyrosine as the horizontal axis, a standard curve was drawn, and the enzyme activity was calculated >30U/mg.
Target Names PROK
Uniprot No. P06873
Alternative Names Endopeptidase K,Tritirachium alkaline proteinase
Species Tritirachium album (Engyodontium album)
Mol. Weight 28.9 kDa
Form Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol.
Note: If you have any special requirement for the glycerol content, please remark when you place the order.
If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.