Loading Control Antibodies and Tag Antibodies
June 28, 2023
1. Loading Control Antibodies
Loading control antibodies are widely used in WB to detect loading control protein expression level and ensure accuracy of experimental results by correcting the error during protein quantitation and sample loading. Besides, loading control can be used as blank control to detect protein transmembrane situation, the staining or ECL process of western blot.
FineTest provides a complete set of polyclonal and monoclonal loading control antibodies with high specificity and wide applications, which can be used in most qualitative, quantitative and localization analysis of proteins. We provide common and rare species loading control antibodies(e.g .human, mouse, rat etc) as well as custom services.
2. How to Choose Loading Control Antibodies?
2.1. Molecular Weight
For an obvious band effect, loading control protein’s MW needs to be >5 KD different from target proteins’ MW.
2.2. Expression Position
Different loading control proteins have different expression levels based on cell organs. We also need to consider sample type while choosing suitable loading control antibodies.
|
Brain |
Spleen |
Skeletal Muscle |
Kidney |
Pancreas |
Testis |
Skin |
Eye |
Heart |
Lung |
α-Tubulin |
√ |
√ |
√ |
√ |
Low |
√ |
√ |
√ |
√ |
√ |
β-Actin |
√ |
√ |
Low |
√ |
√ |
√ |
√ |
√ |
Low |
√ |
β-Tubulin |
√ |
√ |
Low |
√ |
Low |
√ |
√ |
√ |
√ |
√ |
COX IV |
√ |
√ |
√ |
√ |
√ |
√ |
√ |
√ |
√ |
√ |
GAPDH |
√ |
√ |
√ |
√ |
√ |
√ |
√ |
√ |
√ |
√ |
Histone H3 |
√ |
√ |
√ |
√ |
√ |
√ |
Low |
Low |
√ |
√ |
β3-Tubulin |
√ |
Low |
√ |
Low |
√ |
√ |
Low |
√ |
Low |
√ |
**Loading control protein expression level in different mouse tissues.
Sample Type |
Loading Control |
MW(kDa) |
cell membrane |
ATP1A1 |
100-110 |
mitochondrion |
VDAC1 |
31 |
COXIV |
17-20 |
|
endoplasmic reticulum |
calreticulin |
55-60 |
Golgi apparatus |
GM130 |
130 |
**Loading control proteins’ MW and expression level in different cell organs.
Sample Type |
Loading Control |
MW(kDa) |
lysosomes |
LAMP2 |
100-120 |
blood |
Transferrin |
77 |
whole-cell, cytoplasm |
β-actin, α-actin |
42 |
GAPDH |
36 |
|
β-tubulin, α-tubulin |
50-55 |
|
Vinculin (for high MW) |
117 |
|
nucleus |
Lamin B1 |
66 |
TBP |
38 |
|
YY1 |
65-70 |
|
Histone H3 |
18 |
|
PCNA |
38 |
|
HDAC1 |
55 |
3. Advantages of FineTest Antibodies
- A complete set of loading control antibodies, including hot targets, rare species and wide molecular weight range from 15kDa to 130kDa;
- Strict QC and validation, High performance accepted by wide customers;
- Flexible size (small or large) for your choice.
4. Recommended Loading Control Antibodies
Sample Type |
Product Name |
MW(kDa) |
Antibody Type |
Catalog No. |
Applications |
whole-cell, cytoplasm |
anti- beta actin antibody |
42 |
Rabbit pAb |
ELISA, IF, WB, IHC |
|
anti- beta actin antibody |
42 |
Mouse mAb |
ELISA, WB, IHC, IF |
||
anti- GAPDH antibody |
36 |
Rabbit pAb |
ELISA, IHC, WB |
||
anti- GAPDH antibody |
36 |
Mouse mAb |
ELISA, IHC, WB, IF, FC, IP |
||
anti- Beta Tubulin antibody |
50-55 |
Rabbit pAb |
ELISA, WB, IF, IP, IHC, FC |
||
anti- Tubulin-beta antibody |
50-55 |
Mouse mAb |
ELISA, WB, IHC, IF, IP |
**For more loading control antibodies, please contact us for a full list.
5. Tag Antibodies
Recombinant protein usually has a tag during purification. Tag antibody which can specifically recognizes tagged recombinant protein is the efficient research tool to study recombinant tagged protein expression. FineTest offers various tag antibodies strictly validated in applications like WB, IP, IF, Flow Cytometry etc.
5.1. How to Choose Tag Antibodies
Tag antibody needs to be chosen based on the target recombinant proteins’ tag. And we recommend flag tagged protein for WB and Flag, HA or cMyc tag for IP.
5.2. Recommended Tag Antibodies
Tag Type |
Catalog No. |
Applications |
Product Name |
Flag tag |
ELISA, WB, IF |
anti- Flag tag antibody |
|
ELISA, WB, IP |
anti- Flag tag antibody |
||
His tag |
ELISA, WB, IP, IF |
anti- 6*His, His-Tag antibody |
|
ELISA, WB |
anti- 6*his,His tag antibody |
||
HA tag |
ELISA, WB, IP, IF |
anti- HA tag antibody |
|
ELISA, IP, WB, IF |
anti- HA-Tag antibody |
||
Myc tag |
ELISA, WB, IP, IF |
anti- MYC-tag antibody |
|
ELISA, WB, IP, IF |
anti- MYC tag antibody |
**A List of Tag Antibodies.
Tag Type |
Catalog No. |
Applications |
Product Name |
GST tag |
ELISA, WB |
anti- GST Tag antibody |
|
ELISA, WB, IP |
anti- GST tag antibody |
||
GFP tag |
ELISA, WB, IP |
anti- GFP tag antibody |
|
ELISA, WB, IP, IF |
anti- GFP tag antibody |
||
MBP tag |
ELISA, WB, IP |
anti- MBP tag antibody |
|
ELISA, WB |
anti- MBP-Tag antibody |
||
S1 tag |
ELISA, WB |
anti-S1 tag antibody |
|
V5 tag |
ELISA, WB, IP |
anti- V5-tag antibody |
6. Citations
- Article Title: Anti-hyperuricemic effects of three theaflavins isolated from black tea in hyperuricemic mice
IF: 3.197
Journal: Journal of Functional Foods
Cited Product: anti- GAPDH antibody
- Article Title: lncRNA NEAT1 Facilitates Cell Proliferation, Invasion and Migration by Regulating CBX7 and RTCB in Breast Cancer
IF: 3.046
Journal: OncoTargets and Therapy
Cited Product: anti- GAPDH antibody
- Article Title: miR-185-5p response to usnic acid suppresses proliferation and regulating apoptosis in breast cancer cell by targeting Bcl2
IF: 2.482
Journal: Biological Research
Cited Product: anti- beta actin antibody
- Article Title: Inhibition of NLRP3 and Golph3 ameliorates diabetes-induced neuroinflammation in vitro and in vivo
IF: 5.955
Journal: Aging
Cited Product: anti- GOLGA2/GM130 antibody
- Article Title: Expression of gag-CA Gene of Jembrana Disease Virus with Cationic Liposomal and Chitosan Nanoparticle Delivery Systems as DNA Vaccine Candidates
IF: 0
Journal: Tropical Life Sciences Research
Cited Product: anti- GFP tag antibody
- Article Title: MKRN1 Ubiquitylates p21 to Protect against Intermittent Hypoxia-Induced Myocardial Apoptosis
IF: 6.543
Journal: Oxidative Medicine and Cellular Longevity
Cited Product: anti- MYC-tag antibody
- Article Title: Natural variation in WHITE-CORE RATE 1 regulates redox homeostasis in rice endosperm to affect grain quality
IF: 12.796
Journal: The Plant Cell
Cited Product: anti- GST tag antibody, anti- 6*His, His-Tag antibody
- Article Title: Differential Expression of Lonp1 Isoforms in Cancer Cells
IF: 7.666
Journal: Cells
Cited Product: anti- GFP tag antibody